Job Title: Biologist Location: Boston, MA Work schedule: Monday-Friday, 9am-5pm Pay Range: $45 - $52/hr Type: Contract, Potential for extension or conversion to permanent Requirements + Bachelor’s degree in Immunology, Cell Biology, or a related field plus 5 years of relevant experience in biotechnology, pharmaceutical industry, or academic research in the field of immunology. + Master’s degree in Immunology, Cell Biology, or a related field plus 2 years of relevant experience in biotechnology, pharmaceutical industry, or academic research in the field of immunology. Additional Skills/Preferences: + Prior experience with using live cell analysis platforms such as Incucyte and Xcelligence will be a plus + Proficient in developing in vitro assays, including work with human cell lines, PBMCs, and whole blood assays. + Proven ability to utilize immune cell and cytokine analytical techniques, including multiplex ELISA, MSD and flow cytometry + Prior work with CRISPR/Cas9 gene editing techniques. + Prior work with cell culture; Primary and mammalian cell lines + Strong analytical, problem-solving, and decision-making skills. + Excellent interpersonal and communication skills. + Ability to work independently and collaboratively within a team environment Responsibilities + Develop and optimize cell-based assays using primary human immune cells, such as mixed lymphocyte reaction, T-cell, NK-cell cytotoxicity assays, phagocytosis assays, etc. + Isolate and culture different immune cells from either PBMCs or fresh whole blood using immunomagnetic isolation techniques. + Perform differentiation of monocyte to macrophages or dendritic cells, as needed + Assess immune checkpoint receptor expression using multi-parameter flow cytometry techniques + Use different gene editing techniques, including CRISPR to create knockouts or transgenic cell lines + Gene edit different cell types including iPSCs, primary cells, and immortalized cell lines + Use different methods, including cell sorting, single cell cloning to create gene edited clonal cell lines + Design and execute experiments and analyze resulting data to evaluate efficacy of gene edited cells in in vitro assays + Communicate experimental data effectively through written reports and oral presentations. + Demonstrate flexibility and motivation to learn and apply new technologies for biological assays. Benefits System One offers eligible employees health and welfare benefits coverage options, including medical, dental, vision, spending accounts, life insurance, voluntary plans, and participation in a 401(k) plan. Ref: #558-Scientific System One, and its subsidiaries including Joulé, ALTA IT Services, CM Access, TPGS, and MOUNTAIN, LTD., are leaders in delivering workforce solutions and integrated services across North America. We help clients get work done more efficiently and economically, without compromising quality. System One not only serves as a valued partner for our clients, but we offer eligible full-time employees health and welfare benefits coverage options including medical, dental, vision, spending accounts, life insurance, voluntary plans, as well as participation in a 401(k) plan. System One is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex (including pregnancy, childbirth, or related medical conditions), sexual orientation, gender identity, age, national origin, disability, family care or medical leave status, genetic information, veteran status, marital status, or any other characteristic protected by applicable federal, state, or local law.