The Hodges lab is seeking a Research Assistant to participate in a variety of research projects in the area of human epigenomics. Our lab employs state of the art functional genomic techniques, including large-scale DNA sequencing, to study epigenome dynamics in development and disease. About the Work Unit: The epigenome consists of multiple layers that are biochemically modified to affect how genes are regulated. We use cutting-edge DNA sequencing technologies, including ATAC-seq, methylome sequencing, Cut&Run, among others, to map and quantify these epigenetic features throughout the human genome. Using these technologies, we are able to discover previously unknown gene regulatory elements and examine the epigenetic mechanisms that control them. Research in the Hodges lab consists of four major areas that aim to understand the interplay between gene regulatory DNA sequences and epigenetic mechanisms that control gene expression in normal and disease states. Projects in the lab are focused on 1) understanding the mechanistic role of DNA methylation in the cell type specific regulation of gene enhancers and promoters, 2) using comparative DNA methylation profiling to define cell-type essential gene enhancers and trace cell developmental histories, 3) dissecting the relationship between enhancer DNA sequences and epigenetics in human patient data and 4) comparing this relationship across species. Ultimately, our long-term mission is to understand how gene regulatory differences - at both DNA sequence and epigenetic levels - give rise to trait diversity and disease susceptibility. Members of the laboratory are based in recently-renovated space and will work in a collaborative and interactive community space shared by other biochemistry laboratories. The Research Assistant will be a part of the Department of Biochemistry in the School of Medicine at Vanderbilt University and a key laboratory member responsible for duties necessary for maintaining efficient laboratory function, such as preparing stocks, organizing lab supplies, and assisting with various research projects. Key Functions and Expected Performance: Primary Responsibilities include: + Assistance with general laboratory operation, including maintenance of laboratory reagents and stock solutions, placing orders and maintaining lab inventories as well as compliance with Biosafety protocols (50%). + Providing support for specified research projects including data collection, analysis and communication of results (50%). Lab skills will include: + Molecular Biology techniques including DNA subcloning, nucleic acid purification, RT-PCR, quantitative PCR, and production of next generation sequencing libraries. + Cell culture maintenance, including embryonic stem cell culture. + In vitro cell differentiation + Other tasks related to science communication + Attendance at regular meetings with Principal Investigator to discuss laboratory tasks and data + Attendance and participation in regular group laboratory meetings + Proficiency and accuracy in maintaining lab notebook and documentation + Assisting Principal Investigator in training other researchers in lab procedures and protocols Supervisory Relationships: This position does not have any supervisory responsibilities. Education and Certifications: + Academic degree (Bachelor’s) in Biology, Chemistry, or related scientific discipline is required. + Relevant experience in Molecular Biology laboratory techniques and fundamental understanding of chemical principles is strongly preferred. + Ability to adhere to scientific integrity standards in data collection and reporting is necessary. + Ability to understand and follow scientific research protocols and procedures is strongly preferred. + Strong interpersonal and communication skills and the ability to work collaboratively is strongly preferred. + Skill in organizing resources and establishing priorities is strongly preferred. Commitment to Equity, Diversity, and Inclusion At Vanderbilt University, we are intentional about and assume accountability for fostering advancement and respect for equity, diversity, and inclusion for all students, faculty, and staff. Our commitment to diversity makes us who we are. We have created a community that celebrates differences and lets individuality thrive. As part of this commitment, we actively value diversity in our workplace and learning environments as we seek to take advantage of the rich backgrounds and abilities of everyone. The diverse voices of Vanderbilt represent an invaluable resource for the University in its efforts to fulfill its mission and strive to be an example of excellence in higher education. Vanderbilt University is an equal opportunity, affirmative action employer. Women, minorities, people with disabilities, and protected veterans are encouraged to apply.