The Liu lab in the Department of Biophysics and Biophysical Chemistry at SOM is seeking a **_Research Technologist,_** who will perform molecular cloning, cell culture, flow cytometry analysis and reporter gene assays following established protocols and procedures under the supervision of the Research Associate or the PI and contribute to general maintenance of laboratory equipment and supplies. **Specific Duties and Responsibilities** + Work requires basic use of scientific methods, procedures and techniques gained through previous training and experience. Uses basic understanding of theory behind experiments and applies basic analytical skills to implement assigned projects, and to identify and trouble-shoot situations needing special attention. + Follows established procedures or protocols and utilizes aseptic technique to perform laboratory tests and/or experiments. + Perform molecular cloning. + Perform cell culture and maintain HEK293, Hela and BHK-21 cell lines. + Perform flow cytometry, including staining, data acquisition and analysis with FlowJo or similar software. + Assists with reporter gene assays and electrophoretic separations. + Maintains accurate, clear and detailed data records in hard copy and electronically. + Communicate well and work with teams. + Monitors inventory levels, orders materials and supplies through core stores in accordance with established policies and procedures, counts orders upon receipt and makes note in the excel file shared by the lab. **Special Knowledge, Skills, and Abilities** + Understanding of basic experimental design and laboratory safety. + Organizational skills. + Willingness to learn and master new experimental methods and strategies. + Excellent communication skills, willingness to ask questions, and positive attitude. + Technical Qualifications or Specialized Certifications: + Knowledge of Windows computers and Microsoft Office applications. **Specific Physical Requirements** + Occasionally required to traverse throughout the laboratory or to other locations on campus to conduct experiments. + The employee is required to operate computer keyboard, laboratory instruments, and small laboratory tools such as pipettes and/or glassware. + Exposure to toxins and chemicals exists, but potential for personal injury or harm is minimized if established safety and health precautions are followed. + Able to read and understand all Health, Safety, and Environment (HSE) guidelines applicable to assigned work areas. + Use of personal protective equipment may be advised or required. **Minimum Qualifications** + Bachelor's Degree in Biology, Chemistry or related field. + Additional education may substitute for required experience and additional related experience may substitute for required education beyond HS Diploma/Graduation Equivalent, to the extent permitted by the JHU equivalency formula. **Preferred Qualifications** + Some related post-undergraduate work experience preferred. + Familiarity with Microsoft Word, Excel, and PowerPoint software required. Classified Title: Research Technologist Role/Level/Range: ACRO40/E/02/CC Starting Salary Range: $15.70 - $26.25 HRLY ($17,000 targeted; Commensurate w/exp.) Employee group: Part-time Schedule: M-F; 4hrs/day FLSA Status: Non-Exempt Location: School of Medicine Campus Department name: SOM Biophy Biophys and Biophys Chemistry Personnel area: School of Medicine The listed salary range represents the minimum and maximum Johns Hopkins University offers for this position, based on a good faith estimate at the time of posting. Actual compensation will vary depending on factors such as location, skills, experience, market conditions, education, and internal equity. Not all candidates will qualify for the highest salary in the range. Johns Hopkins provides a comprehensive benefits package supporting health, career, and retirement. Learn more: https://hr.jhu.edu/benefits-worklife/. Equal Opportunity Employer All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran. EEO is the Law https://www.eeoc.gov/sites/default/files/2023-06/22-088_EEOC_KnowYourRights6.12ScreenRdr.pdf