Why Sarepta? Why Now?
The promise of genetic medicine has arrived, and Sarepta is at the forefront. We hold leadership positions in Duchenne muscular dystrophy (DMD) and limb-girdle muscular dystrophies (LGMDs), and we currently have more than 40 programs in various stages of development in gene therapy, RNA and gene editing. In 2023, we launched our fourth therapy and the first ever gene therapy to treat Duchenne.
We’re looking for people who see unlimited potential in themselves and who are motivated by an unwavering commitment to patients.
What Sarepta Offers
At Sarepta, we care deeply about all the people in our community and believe in the importance of supporting them in all aspects of their lives. We aspire to maintain a culture that acknowledges people bring their whole selves to work, and we will strive to help everyone in our community integrate their work and personal lives while maintaining productivity. We are committed to offering a range of benefits and work-life resources designed to support people in the following areas:
Physical and Emotional Wellness
Financial Wellness
Support for Caregivers
For a full list of our comprehensive benefits, see our website: https://www.sarepta.com/join-us
The Importance of the Role
This individual will join a team of scientists to support the predevelopment of cell based AAV gene therapy potency assay methods at the Sarepta Andover facility. The position requires a strong background in cell culture and experience in cell-based assay method development. The individual needs demonstrable experience in managing cell lines, both wild type and KO cell lines. The candidate should have hands on experience of SDS-PAGE, qPCR, ddPCR, protein extraction methods, antibody screening, and protein quantification methods using recombinant protein. The individual will be responsible to support analytical method development. This person will interface with the internal and external cross-functional teams.The Opportunity to Make a Difference
Hands on experience with cell culture to create and maintain unique mammalian wild type and KO cell lines for cell-based assays. Testing new cell lines for use in potency assays for multiple new gene therapy programs.Managing the production, screening, and testing multiple recombinant protein standards and antibodies for multiple target proteins under several gene therapy programsConduct laboratory experiments involving in vitro potency methods to select the suitable cell lines, antibody and recombinant proteins.Redesign multiple cell lines to enhance the expression of surface receptors in a reproducible manner to increase infectabilityCreate and maintain clear and concise lab records and documentation.Author test methods, predevelopment reports, and study protocols.Support the existing development team, transfer and qualification/validation activitiesAct as company liaison with CMOs to coordinate in method development and method transfer across various CMO/CRO sites as neededMore about You
Ph.D in molecular biology, cell biology or virology with at least 1+ years of industrial experience.Working experience in a cGMP environment is preferredA strong understanding of AAV related cell culture methods, capillary based electrophoresis, molecular cloning, AAV based characterization method is necessary.Experience in general laboratory experimentation, documentation with adherence to cGMP is necessary.Experience in developing, optimizing and transferring molecular biology test methods is useful.Must have effective written and verbal communication skillsOperate as part of a team; assisting fellow lab associates in providing a functional, safe working environment while maximizing laboratory efficiencyWhat Now?
We’re always looking for solution-oriented, critical thinkers.
So, if you’re comfortable with ambiguity and candor, relish challenging yourself, and place kindness and integrity at the forefront of how you approach your peers and work, then we encourage you to apply.