Description

Experimental

Nuclei isolation and fluorescence activated nuclei/cell sorting (FANS/FACS). Preparation of single-cell methylome (snmC-seq2) and multi-omic (sn-m3C-seq, snmCT-seq) libraries using a robotic liquid handler system (Beckman Biomek i7). Prepare buffers and solutions. Manage the inventory of reagents and tissue samples used for single-cell experiments. Perform quality control analyses of high-throughput sequencing libraries. Accept responsibility for performance of all laboratory procedures under the direction of supervisors. Keep an up-to-date standard operating procedures (SOPs). Maintain freezers and refrigerators by periodic thawing and cleaning and organizing their contents Manage biohazard and recycle wastes. Ensure adequate supply of liquid nitrogen for cell storage and CO2 for tissues culture. Monitor equipment function and report and arrange for repair of malfunctioning equipment. Clean the tissue culture hood, incubator and surfaces as well as pipettes and pipet aids as needed.

Administrative & General Laboratory Duties

Help organize the laboratory and maintain general cleanliness. Attend group lab meetings, and implementations of protocols. Abide by any procedural changes, policy changes, laboratory safety and general information. Inform principle investigator of personnel problems, difficulties with procedures, equipment or other matters as needed. Notify the supervisor of any need for the inventory and ordering of reagents and supplies. Maintain all laboratory certificates and permit use including preparing new or renewal applications When needed, place orders for the members of the lab with appropriate documentation upon receipt of ordered material.