The Staff Scientist will be part of the Biochemistry department at Vanderbilt University. The overall goal of the lab is to determine the molecular mechanisms regulating development of the peripheral nervous system. This position will help maintain day to day functions of the lab, help establish new experimental protocols, and participate in novel research. This position will require both independent and collaborative work, so the abilities to learn new techniques, self-manage projects, and work in a team is essential. This position includes opportunities for publication and laboratory experience that can provide a strong background for future applications to advanced degree programs.

About the Work Unit:
The research laboratory in the department of Biochemistry at Vanderbilt University investigates the regulation of peripheral myelin formation by Schwann cells and how this process is disrupted in Charcot Marie Tooth disease. The primary techniques to achieve this will be transgenic mouse breeding, primary cell culture, dissection of rodent nerves and neurons, general biochemistry and molecular biology, cell imaging, and some -omics analysis. The work unit is made up of faculty, research staff, postdoctoral
fellows, and graduate students.
 

Key Functions and Expected Performance:
Assists in Research and Development:
     • Researches and collects data using appropriate lab experiments, procedures, and techniques
     • Preparation of buffers and reagents
     • Production and maintenance of DNA vectors, mammalian cell lines, and virus stocks
     • Production and purification of virus and virus-like particles using mammalian cell culture
     • Preparation of primary Schwann cell and neuron cultures
     • Assist in maintaining and breeding transgenic mouse lines
     • Collects, analyzes, and interprets data with assistance of other members of the laboratory
     • Writes and updates laboratory protocols
     • Reports on status of research activities in oral and written formats
     • Assembles and compile research data for presentation and/or reports
Maintenance
     • Conducts general lab maintenance
     • Assists in set up, operation, and maintenance of laboratory equipment including, but not limited to – biosafety cabinets, incubators, centrifuges, etc.
     • Proper storage and maintenance of DNA and virus stocks

Performs administrative duties
     • Monitors inventory of supplies and assists with ordering
     • Participate in weekly and bimonthly meetings to discuss project progress
     • Other tasks as directed by supervisor

Supervisory Relationships:
     • This position will supervise undergraduate students, graduate students who are rotating and new research assistants.
     • The position reports administratively and functionally to the PI.

Education and Certifications:
     ▪ A Bachelor’s degree in biochemistry, biological sciences, chemistry, or related field from an accredited institution of higher education is necessary.

Experience and Skills:
     ▪ Knowledge and experience with mammalian cell culture and protein expression is preferred.
     ▪ Knowledge of virus expression and purification is preferred.
     ▪ Knowledge of fluorescence microscopy is preferred.
     ▪ Experience with breeding, genotyping and handling mice is preferred.
     ▪ Experience with organization, inventory, and record keeping is preferred.
     ▪ Excellent organizational and communications skills (both verbal and written) are preferred.